TransFast Taq DNA Polymerase(with 2.5 mM dNTPs)


TransFast Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.

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Features

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of Tgenomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• High throughput PCR
• Colony PCR

Storage

at -20 ℃ for two years

 

Product Composition

Component    AP101-01/11    AP101-02/12
TransFast ® Taq DNA Polymerase           500 U×1           500 U×6
10×TransFast ® Taq Buffer          1.2 ml ×1          1.2 ml ×6
2.5 mM dNTPs     – / 800 μl ×1     – / 800 μl ×6
6×DNA Loading Buffer              1 ml×1             1 ml ×2
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