TransTaq-T DNA Polymerase


TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.

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Features

• TransTaq-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq DNA Polymerase.

• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

Applications

• Complex templates
• TA cloning

Storage

at -20 ℃ for two years

 

Product Composition

Component   AP122-01/11   AP122-02/12   AP122-03/13
TransTaq ®-T DNA Polymerase          250 U×1          500 U×1          500 U×6
10×TransTaq ®         1.2 ml ×1         1.2 ml ×1         1.2 ml ×6
2.5 mM dNTPs    – / 400 μl ×1    – / 800 μl ×1    – / 800 μl ×6
6×DNA Loading Buffer          500 μl×1            1 ml ×1            1 ml ×2
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