Chemicals & Reagents

TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransStart^® FastPfu DNA Polymerase is a hot start high-fidelity DNA polymerase used for fast PCR. Offering a high amplification efficiency and high extension rate (4 kb/min, it is 8 times that of ordinary Pfu enzymes), TransStart^® FastPfu DNA Polymerase amends the defects of low amplification efficiency, low yield and low extension rate (0.5 kb/min) in common Pfu polymerase, and greatly shortens reaction time.

TransTaq DNA Polymerase High Fidelity (TransTaq HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.