Laboratory

TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.

TransStart^® FastPfu DNA Polymerase is a hot start high-fidelity DNA polymerase used for fast PCR. Offering a high amplification efficiency and high extension rate (4 kb/min, it is 8 times that of ordinary Pfu enzymes), TransStart^® FastPfu DNA Polymerase amends the defects of low amplification efficiency, low yield and low extension rate (0.5 kb/min) in common Pfu polymerase, and greatly shortens reaction time.

TransStart® FastPfu Fly DNA Polymerase is a hot start, ultra-high-fidelity DNA Polymerase used for fast PCR. Compared withTransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has a higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity, and higher specificity. 2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water, and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of the Reaction Mix being 1×. PCR products are blunt-ended and can be cloned directly into the pEASY®-Blunt series of vectors.

TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

TransTaq-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.

TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.