EasyTaq PCR SuperMix is a ready-to-use mixture of EasyTaq DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. For 2×EasyTaq PCR SuperMix (-dye), the PCR products which contain dA overlangs at the 3′ end can be directly cloned to pEASY®-T vectors. For 2×EasyTaq® PCR SuperMix (+dye), the PCR products can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning.
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• Reduced reaction setup time
• Minimized contamination caused by multi-step operation.
• High amplification efficiency and stability.
• Amplification of genomic DNA fragment up to 4 kb.
Routine PCR
at -20 ℃ for two years
| Component | AS111-01/11 | AS111-02/12 | AS111-03/13 | AS111-14 |
| 2×EasyTaq® PCR SuperMix (-dye)/(+dye) | 1 ml | 5×1 ml | 15×1 ml | 6×80 ml |
| Nuclease-free Water | 1 ml | 5 ml | 3×5 ml | 96×5 ml |
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