2?TransStart GoldPfu PCR SuperMix (-dye)


This product is a ready-to-use mixture of TransStart GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra-high fidelity, and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis.

Compare
  • Reduced PCR operation time.
  • Avoid contamination due to multi-step operation.
  • 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Amplification of plasmid DNA fragment up to 20 kb.

 

Highlights

Fast amplification, ultra-high fidelity, high specificity, high stability

Applications

  • Ultra High Fidelity PCR
  • Site-directed mutagenesis
  • Blunt-end cloning
  • Complex templates
  • GC/AT-rich templates
  • Long fragment PCR

Storage

at -20 ℃ for two years

Shipping

Dry ice (-70 ℃)

Product Composition

Component AS401-01 AS401-02 AS401-03
TransStart® GoldPfu PCR SuperMix (-dye)         1 ml     5×1 ml   15×1 ml
Nuclease-free Water         1 ml         5 ml     3×5 ml
en English