2?TransTaq? High Fidelity (HiFi) PCR SuperMix I (-dye)


TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs, and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA, and PCR SuperMix II is optimized for the amplification of λDNA, cDNA, or plasmid DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, and H2O.

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Features

• TransTaq High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 1-2 kb/min.

• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 15 kb.

 

Applications

• Complex templates

• GC/AT-rich templates

• Long PCR

• High yield PCR

 

Storage

at -20 ℃ for two years

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