2?TransTaq-T PCR SuperMix (-dye)

2?TransTaq-T PCR SuperMix (-dye)

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TransTaq-T PCR SuperMix is a ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. Efficiency of PCR products with “A” is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.

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Features

• TransTaq-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

Applications

• Complex templates
• TA cloning

Storage

at -20 ℃ for two years

Product Composition

Component AS122-01/21 AS122-02/22
2×TransTaq®-T PCR SuperMix (-dye)/(+dye)              1 ml          5×1 ml
Nuclease-free Water              1 ml               5 ml
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