The ARACUS „advanced“ Amino Acid Analyzer is an extended configuration of the ARACUS „classic“ by an additional second pump drive. The upgraded pump configurations allows the independent operation of two fluidic lines (eluent side and reactive side).The use of two pump drives results in an improved signal-to-noise ratio of the chromatogram. The freely adjustable parameters between the eluent and the reagent pump drives also provide the possibility of using the reagent OPA (o-phthalaldehyde) for detection of primary amines with a fluorescence detector.
Technical Specifications of ARACUS “advanced” Amino Acid Analyzer
Method
- post column derivatization with Ninhydrin
Autosampler
- 2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 – 50 μL), microliter pickup (1 – 35 μL)
Column
- stainless steel, cation exchange resin reproducibility of retention time <1 % RSD, reproducibility of peak area < 3.0 % RSD
Column Oven
- 20 – 100 °C, accuracy 0.1 %
Reactor Temperature
- 20 – 140 °C
Spectroscopic detection
- measuring range: high, low LED photometers (440 nm + 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty
Detection limit
- < 40 pmol / 20 µL with Ninhydrin, < 4 pmol / 20 µL with OPA
Quantification limit
- > 100 pmol / 20 µL with Ninhydrin, > 10 pmol / 20 µL with OPA
Pump system
- dual pump reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 – 10.00 mL/min
Maximum operating pressure
- 120 bar
Ready to use kits
- kits including eluents, reagent and additional dilution buffer, ammonia absorber and separation columns for 500 – 1000 runs
Columns & Eluents
- Compatible with all columns, operating with all eluents based on different ionic strength (Li, Na, K)
Control software & Data acquisition
- Clarity (21 CFR part 11 compliant) or AminoPeak
Power
- 110 – 230 V
Dimensions
- 1000 mm x 680 mm x 360mm
Weight
- 60 kg
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