Double-Through Separating Chamber


The Double-Through Separating Chamber is designed for chromatography requiring low or dual-solvent use in a single chamber. It minimizes solvent consumption, making the process cost-effective and environmentally friendly. Pre-equilibration ensures reproducible chromatography results.

The Double-Through Separating Chamber enables simultaneous TLC development of two plates with identical or different solvents. Ideal for comparison studies, quality control, and dual-sample analysis in pharmaceutical, forensic, or research laboratories.

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The Double-Through Separating Chamber is a versatile, dual-compartment TLC development chamber designed for the simultaneous development of two thin-layer chromatography plates. Built from durable, high-quality borosilicate glass or chemically resistant polymer, this chamber enhances laboratory efficiency by allowing side-by-side comparison of samples or methods.

The chamber features two separate internal grooves or slots that securely hold two TLC plates—commonly up to 20×20 cm or 10×20 cm in size. Each compartment functions independently, permitting either the same or two different solvent systems to be used simultaneously. This feature makes it ideal for comparative chromatographic studies, solvent system evaluation, or parallel sample analysis.

A precision-sealed lid—either ground-glass or flat—ensures a tight seal, minimizing solvent evaporation while maintaining an evenly saturated internal atmosphere. This uniform saturation guarantees consistent development across both plates, resulting in sharp, reproducible separations.

The clear design of the Double-Through Separating Chamber allows users to monitor solvent front progression in both compartments, facilitating real-time observation and accurate timing for plate removal. Whether for pharmaceutical quality control, forensic testing, environmental screening, or food safety analysis, this chamber supports efficient and reliable dual-sample workflows.

In research and development settings, the double-through design saves time and solvents by enabling comparative experiments within the same device. Academic institutions also benefit, using the chamber to teach chromatography principles and demonstrate solvent effects in a single lab session.

Reusable and easy to clean, the Double-Through Separating Chamber is built for long-term use, providing exceptional value and performance. Its ability to handle simultaneous TLC development with either matched or varied solvent systems sets it apart as a highly practical and innovative tool for modern chromatographic analysis.

Features of Double-Through Separating Chamber

  • Chamber Type: Dual-compartment TLC development chamber

  • Material: Made from high-quality borosilicate glass or chemically resistant plastic

  • Capacity: Typically supports two TLC plates simultaneously (commonly 20×20 cm or 10×20 cm)

  • Double-Slot Design: Two internal tracks or grooves allow separate yet parallel development of plates

  • Independent Solvent Tracks: Enables development with the same or different solvent systems concurrently

  • Tight-Sealing Lid: Ground-glass or flat lid ensures airtight sealing for minimal solvent evaporation

  • Uniform Saturation: Each compartment offers even solvent vapor distribution for consistent development

  • Clear Construction: Transparent walls for easy visualization of both plates

  • Reusable: Durable and long-lasting, suitable for frequent lab use

  • Efficient Workflow: Allows simultaneous analysis, improving throughput in QC and research labs

Applications of Double-Through Separating Chamber

  1. Simultaneous Plate Development

    • Run two samples side-by-side using identical or different solvent systems.

  2. Pharmaceutical Quality Control

    • Double-check drug purity or formulation comparisons with dual TLC setups.

  3. Comparative Analysis in R&D

    • Compare reaction pathways or test two compounds under varied conditions in one go.

  4. Educational Use

    • Demonstrate chromatographic effects of different solvents simultaneously.

  5. Forensic Testing

    • Analyze a reference and test sample in the same chamber for accuracy.

  6. Environmental and Food Testing

    • Efficient for testing multiple pollutant or contaminant samples in parallel.

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