EasyTaq DNA Polymerase for PAGE

EasyTaq DNA Polymerase for PAGE

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EasyTaq DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.

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Features

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.

Applications

Short fragment PCR

Storage

at -20 ℃ for two years

 

Product Composition

Component  AP112-01/11  AP112-02/12
EasyTaq ® DNA Polymerase for PAGE           2500 U       2500 U×4
10×EasyTaq ® Buffer for PAGE       1.2 ml ×5       1.2 ml×20
2.5 mM dNTPs   – / 800 μl×5  – / 800 μl×20
6×DNA Loading Buffer               1 ml          1 ml ×4
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