EasyTaq DNA Polymerase (with 2.5 mM dNTPs)


EasyTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

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Features

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• High throughput PCR
• Colony PCR

Storage

at -20 ℃ for two years

 

Product Composition

Without dNTPs

Component     AP111-01    AP111-02     AP111-03     AP111-04
EasyTaq ® DNA Polymerase      500 U×1     500 U×6    2500 U×4   5000 U×10
10×EasyTaq ® Buffer      1.2 ml×1     1.2 ml×6    1.2 ml×20   1.2 ml×100
6×DNA Loading Buffer         1 ml×1        1 ml×2         1 ml×4        1 ml×20

With dNTPs

Component      AP111-11     AP111-12     AP111-13
EasyTaq ® DNA Polymerase       500 U×1      500 U×6    2500 U×4
10×EasyTaq ® Buffer       1.2 ml×1      1.2 ml×6    1.2 ml×20
2.5 mM dNTPs       800 μl×1     800 μl ×6    800 μl×20
6×DNA Loading Buffer          1 ml×1         1 ml×2        1 ml×4
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