Phosphorylation – Cartridge


The Phosphorylation Cartridge is a reagent cartridge designed for the Kilobaser one-XT DNA/RNA Synthesizer, enabling the synthesis of DNA oligonucleotides with a 5′-phosphate group. This modification is essential for applications involving DNA ligation and other molecular biology techniques.​

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Key Features

  • Reagent Volume: Sufficient for synthesizing 100 DNA bases, with 75 nucleotides each of A, T, C, and G. ​

  • Modification: Incorporates a 5′-phosphate group, facilitating ligation and other enzymatic reactions.

  • Compatibility: Exclusively compatible with the Kilobaser one-XT synthesizer and requires the Phosphorylation Chip for labeled oligo synthesis.

  • Shelf Life: Stable for up to 1 year when stored at -20°C; recommended to use within 1 week after opening. ​

  • Safety: Closed system design eliminates direct contact with chemicals, enhancing user safety.​

Synthesis Output

The number of oligonucleotides you can synthesize depends on their length:​

  • 4 oligos of 25 bases each​

  • 5 oligos of 20 bases each​

Applications

  • Reagent volume for 100 DNA bases

  • 75 nucleotides each of A, T, C and G

  • Shelf life of 1 year

  • Store at -20°C

  • Use within 1 week of opening

  • No additional reagents needed

  • For research and development only, no therapeutic applications

  • This product is for use with Kilobaser one-XT only

Ideal for creating:​

  • Ligation-Ready DNA Fragments: Facilitates the joining of DNA fragments in cloning and other molecular biology workflows.​

  • Enzymatic Reactions: Suitable for applications requiring 5′-phosphorylated DNA, such as kinase assays.​

  • Diagnostic Assays: Useful in developing assays that require phosphorylated DNA strands.​

Specifications

Download Specification sheet

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