Phosphorylation – Chip


The Phosphorylation Chip is a single-use microfluidic chip designed for the Kilobaser one-XT DNA/RNA Synthesizer, enabling the synthesis of DNA oligonucleotides with a 5′-phosphate group. This modification is essential for applications involving DNA ligation and other molecular biology techniques.​

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Key Features

  • Modification: Incorporates a 5′-phosphate group into DNA oligonucleotides.

  • Compatibility: Designed for use with the Kilobaser one-XT synthesizer and the Phosphorylation DNA Cartridge.

  • Usage: Single-use; one chip required per synthesis.

  • Shelf Life: Stable for up to 1 year when stored dry at room temperature.

  • Disposal: Can be disposed of with regular laboratory waste.

Applications

  • For 5′-end phosphorylated DNA oligo synthesis

  • Shelf life of 1 year

  • Store dry at room temperature

  • Do not remove the chip’s foil!

  • One disposable chip is needed per synthesis

Ideal for synthesizing:

  • Ligation-Ready DNA Fragments: Facilitate the joining of DNA fragments in cloning and other molecular biology workflows.

  • Enzymatic Reactions: Suitable for applications requiring 5′-phosphorylated DNA, such as kinase assays.

  • Diagnostic Assays: Useful in developing assays that require phosphorylated DNA strands.

Specifications

Download Specification sheet

 

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