T4 DNA Polymerase

T4 DNA Polymerase:
High-Fidelity Enzyme for DNA Blunting, Repair, and Processing in Molecular Biology

T4 DNA Polymerase is a highly versatile, double-stranded DNA-modifying enzyme derived from the T4 bacteriophage. It exhibits strong 3’→5′ exonuclease and 5’→3′ polymerase activities, making it a powerful tool for various DNA end-processing applications in molecular cloning, next-generation sequencing (NGS) preparation, and genetic engineering workflows.

This enzyme is primarily used to convert DNA overhangs into blunt ends, fill in recessed 3′ ends, or remove protruding 3′ termini, ensuring clean, uniform DNA fragments ready for ligation or further enzymatic manipulation.

Key Features and Benefits

Dual Enzymatic Activity

• 5’→3′ Polymerase Activity allows for filling in 5′ overhangs.

• 3’→5′ Exonuclease Activity enables removal of 3′ overhangs, ensuring blunt-ended DNA.

Ideal for Blunt-End Cloning
Perfect for converting sticky ends to blunt ends for cloning, ligation into blunt-end vectors, or preparing DNA for NGS workflows.

High Purity and Specificity
Manufactured under stringent quality control, ensuring high enzyme activity and minimal contamination from host nucleases or DNA.

Fast Reaction Time
Efficient processing within minutes under optimized buffer conditions—compatible with a wide range of DNA substrates and reaction volumes.

Stable and Reliable Performance
Delivered with a compatible reaction buffer and optimized for consistent results across routine and advanced molecular biology applications.

Scalable for Various Workflows
Available in multiple unit sizes for small lab use or high-throughput processing needs in research and biotech industries.

Applications

• End-polishing of DNA for blunt-end cloning

• DNA repair and preparation for sequencing

• Site-directed mutagenesis

• Fill-in of 5′ overhangs

• Removal of 3′ protruding ends

• Adapter ligation workflows

• Molecular diagnostics and gene editing preparation

T4 DNA Polymerase is a trusted tool in modern molecular biology, offering reliable and high-fidelity DNA end-processing capabilities. Whether you’re constructing recombinant plasmids, preparing samples for sequencing, or performing DNA repair, this enzyme delivers the precision and quality your work demands.