TransFast Taq DNA Polymerase


TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.

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Features

• Extension rate is about 6 kb/min.

• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• High throughput PCR
• Colony PCR

Storage

at -20 ℃ for two years

 

Product Composition

Component     AP101-01/11     AP101-02/12
TransFast® Taq DNA Polymerase            500 U×1            500 U×6
10×TransFast® Taq Buffer           1.2 ml ×1           1.2 ml ×6
2.5 mM dNTPs      – / 800 μl ×1      – / 800 μl ×6
6×DNA Loading Buffer               1 ml×1              1 ml ×2
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