• Offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Blunt-end PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb
Features
• Hot start, high specificity.
• High amplification efficiency.
• Fast and high fidelity.
Applications
• Amplifies complex and high GC/ AT templates.
• High fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.
• Amplifies long fragment.
Product Composition
| Component | AP221-01/11 | AP221-02/12 | AP221-03/13 |
| TransStart® FastPfu DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 5×TransStart® FastPfu Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | – / 500 μl×1 | – / 1 ml ×1 | – / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 200 μl×1 | 1 ml ×1 | 1 ml ×2 |
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