TransStart FastPfu Fly DNA Polymerase


TransStart® FastPfu Fly DNA Polymerase is a hot start, ultra-high-fidelity DNA Polymerase used for fast PCR. Compared withTransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has a higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity, and higher specificity. 2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water, and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of the Reaction Mix being 1×. PCR products are blunt-ended and can be cloned directly into the pEASY®-Blunt series of vectors.

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Features

• Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

• PCR products can be directly cloned into pEASY ®-Blunt vectors.

• Amplification of genomic DNA fragment up to 15 kb.

• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• Complex templates, GC/AT-rich templates.

• Ultra high-fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.

• Long fragment amplification.

Storage

at -20 ℃ for two years

 

Product Composition

Component    AP231-21-V4    AP231-22-V4    AP231-23-V4
TransStart® FastPfu Fly DNA Polymerase            250 U×1           500 U×1           500 U×6
TransStart® FastPfu Fly Reaction Mix              1 ml×3          1.2 ml ×5        1.2 ml ×30
50 mM MgSO4           200 μl×1          400 μl ×1             1 ml ×1
6×DNA Loading Buffer           500 μl×1             1 ml ×1             1 ml ×2
PCR Stimulant           200 μl×1           400 μl×1             1 ml ×1
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