Features
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage
at -20 ℃ for two years
Product Composition
| Component | AP301-01/11 | AP301-02/12 | AP301-03/13 |
| TransStart® KD Plus DNA Polymerase | 100 U×1 | 200 U×1 | 200 U×6 |
| 5×TransStart® KD Plus Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | – / 500 μl×1 | – / 1 ml ×1 | – / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
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