TransTaq DNA Polymerase High Fidelity (HiFi) (with 2.5 mM dNTPs)


TransTaq DNA Polymerase High Fidelity (TransTaq HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

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Features

• TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR

Storage

at -20 ℃ for two years

 

Product Composition

Component    AP131-01/11    AP131-02/12    AP131-03/13
TransTaq® HiFi DNA Polymerase           250 U×1           500 U×1           500 U×6
10×TransTaq® HiFi Buffer I          1.2 ml×1           1.2 ml×1           1.2 ml×6
10×TransTaq® HiFi Buffer II          1.2 ml×1           1.2 ml×1           1.2 ml×6
2.5 mM dNTP       -/400 μl×1        -/800 μl×1        -/800 μl×6
10×GC Enhancer          200 μl×1           400 μl×1              1 ml×1
6×DNA Loading Buffer          500 μl×1              1 ml×1              1 ml×2
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